一款在细胞内或活体动物体内乏氧部位的荧光示踪产品CY7-NO2

杭州新乔生物可以提供2种荧光示踪产品可以用于细胞内或活体体内乏氧部位的荧光示踪CY7-NO2

Selective and efficient detection andimaging of nitroreductase (NTR) overexpressed in hypoxic tissues is of great importancefor better understanding its biological functions. The effective optical probesequipped for NTR detection in vivo is still lacking, so we developed aninnovative near infrared (NIR) fluorescent on-off probe (Cy-NO2) composed offluorescence reporting unit (an aminocyaine dye) and recognition unit (p-nitrobenzylcarbamategroup). The response and mechanism of the NTR activated reduction of Cy-NO2were evaluated in vitro through kinetic optical studies, mass spectra analysisand docking calculations. The results demonstrated that Cy-NO2 could rapidlyrecognize NTR with high selectivity and sensitivity. The efficient NTR

detection ability of Cy7-NO2 was furthervalidated by fluorescence imaging of hypoxic cells (A549, PC-12 and HUVECcell). Furthermore, the in vivo hypoxia imaging by Cy-NO2 were evaluated on tumor-bearingmouse,cerebral ischemia (CIS) and deep vein thrombosis models. The resultsindicated a rapid and distinct enhancement of its NIR fluorescence highlyappropriate for in vivomonitoring of NTR in all of the three animal models,whichrevealed aspiring clinical value of this NIR fluorescent hypoxia probe.